DNA

Part:BBa_K2100044:Design

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-17)


pEXPR pERE6:BM3R1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 18
    Illegal EcoRI site found at 333
    Illegal EcoRI site found at 343
    Illegal EcoRI site found at 749
    Illegal XbaI site found at 42
    Illegal PstI site found at 566
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 18
    Illegal EcoRI site found at 333
    Illegal EcoRI site found at 343
    Illegal EcoRI site found at 749
    Illegal NheI site found at 983
    Illegal PstI site found at 566
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 18
    Illegal EcoRI site found at 333
    Illegal EcoRI site found at 343
    Illegal EcoRI site found at 749
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 18
    Illegal EcoRI site found at 333
    Illegal EcoRI site found at 343
    Illegal EcoRI site found at 749
    Illegal XbaI site found at 42
    Illegal PstI site found at 566
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 18
    Illegal EcoRI site found at 333
    Illegal EcoRI site found at 343
    Illegal EcoRI site found at 749
    Illegal XbaI site found at 42
    Illegal PstI site found at 566
    Illegal NgoMIV site found at 488
    Illegal NgoMIV site found at 723
    Illegal AgeI site found at 501
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

This is a synthetic promoter with a gene from the mammalian genome.

References