DNA
Part:BBa_K2100044:Design
Designed by: Kathleen Brandes Group: iGEM16_MIT (2016-10-17)
pEXPR pERE6:BM3R1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 18
Illegal EcoRI site found at 333
Illegal EcoRI site found at 343
Illegal EcoRI site found at 749
Illegal XbaI site found at 42
Illegal PstI site found at 566 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 18
Illegal EcoRI site found at 333
Illegal EcoRI site found at 343
Illegal EcoRI site found at 749
Illegal NheI site found at 983
Illegal PstI site found at 566 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 18
Illegal EcoRI site found at 333
Illegal EcoRI site found at 343
Illegal EcoRI site found at 749 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 18
Illegal EcoRI site found at 333
Illegal EcoRI site found at 343
Illegal EcoRI site found at 749
Illegal XbaI site found at 42
Illegal PstI site found at 566 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 18
Illegal EcoRI site found at 333
Illegal EcoRI site found at 343
Illegal EcoRI site found at 749
Illegal XbaI site found at 42
Illegal PstI site found at 566
Illegal NgoMIV site found at 488
Illegal NgoMIV site found at 723
Illegal AgeI site found at 501 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
Source
This is a synthetic promoter with a gene from the mammalian genome.